Detailed view for TcCLB.511649.150

Basic information

TDR Targets ID: 37466
Trypanosoma cruzi, phosphoglycerate mutase protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.3573 | Length (AA): 185 | MW (Da): 20849 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00300   Histidine phosphatase superfamily (branch 1)

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 184 1bif () 249 421 25.00 0 1 1.31 -0.61
2 183 1h2e (A) 3 182 27.00 0 1 1.44 -1.02
1 108 5um0 (A) 1 110 39.00 0.000000011 0.99 1.06318 -0.3
2 183 1h2e (A) 3 182 27.00 0.00000000057 1 1.38738 -0.68
4 161 1fbt (A) 3 151 30.00 0 1 1.22715 -0.53
46 113 3fjy (A) 230 297 18.00 0 0.04 0.612668 -0.81

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129378)

Species Accession Gene Product
Arabidopsis thaliana AT5G04120   phosphoglycerate mutase-like protein
Arabidopsis thaliana AT3G50520   phosphoglycerate mutase
Candida albicans CaO19.13477   similar to phosphoglycerate mutases
Candida albicans CaO19.6056   similar to phosphoglycerate mutases
Drosophila melanogaster Dmel_CG7059   CG7059 gene product from transcript CG7059-RA
Drosophila melanogaster Dmel_CG15874   Phosphoglycerate mutase 5-2
Escherichia coli b4395   phosphatase
Leishmania braziliensis LbrM.08.0060   phosphoglycerate mutase protein, putative
Leishmania donovani LdBPK_080060.1   phosphoglycerate mutase protein, putative
Leishmania infantum LinJ.08.0060   phosphoglycerate mutase protein, putative
Leishmania major LmjF.08.0060   phosphoglycerate mutase protein, putative
Leishmania mexicana LmxM.08.0060   phosphoglycerate mutase protein, putative
Mycobacterium leprae ML1452c   PROBABLE PHOSPHOGLYCERATE MUTASE (PHOSPHOGLYCEROMUTASE)
Mycobacterium tuberculosis Rv2228c   Multifunctional protein. Has RNASE H, alpha-ribazole phosphatase, and acid phosphatase activities.
Mycobacterium ulcerans MUL_3691   phosphoglycerate mutase
Neospora caninum NCLIV_034520   Phosphoglycerate/bisphosphoglycerate mutase (EC 5.4.2.1), related
Oryza sativa 4349723   Os11g0138600
Saccharomyces cerevisiae YOR283W   phosphoglycerate mutase
Trypanosoma brucei gambiense Tbg972.5.5000   phosphoglycerate mutase protein, putative
Trypanosoma brucei Tb927.5.3580   phosphoglycerate mutase protein, putative
Trypanosoma congolense TcIL3000_0_40660   phosphoglycerate mutase protein, putative
Trypanosoma congolense TcIL3000_5_4010   phosphoglycerate mutase protein, putative
Trypanosoma cruzi TcCLB.511649.150   phosphoglycerate mutase protein, putative
Trypanosoma cruzi TcCLB.507811.44   phosphoglycerate mutase protein, putative
Toxoplasma gondii TGME49_273030   phosphoglycerate mutase family protein
Trichomonas vaginalis TVAG_165570   phosphoglycerate mutase, putative

Essentiality

TcCLB.511649.150 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.3580 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.3580 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.3580 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.5.3580 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b4395 Escherichia coli non-essential goodall
TGME49_273030 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Phosphoglycerate Mutase (5.4.2.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

  • Tcru023995AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tcru023995; Recombinant protein: full-length; Source: T cruzi; 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (ec 5.4.2.1)(phosphoglyceromutase) (pgam) (bpg-dependent pgam) (dpgm), putative ;

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.511649.150 (Trypanosoma cruzi), phosphoglycerate mutase protein, putative
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